February 23, 2011Virology JournalOpen Access

A pseudotype baculovirus expressing the capsid protein of foot-and-mouth disease virus and a T-Cell immunogen shows enhanced immunogenicity in mice

Q: What is the clinical evidence from this study?

Study design: Other. Population: Foot-and-mouth disease (FMD) vaccination model (n=56). Intervention: Bac-GED-P12A3CT (recombinant pseudotype baculovirus with T-cell epitopes) vs. Bac-GED-P12A3C (without T-cell epitopes). Primary outcome: IFN-gamma production (pg/ml) in splenocytes at 7 weeks (1 × 10^10 PFU dose) (p=<0.01).

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Key result

Recombinant pseudotype baculovirus with T-cell epitopes significantly enhanced IFN-gamma production (P < 0.01) and neutralizing antibody titers (P < 0.05) compared to the recombinant without T-cell epitopes in mice.

Population

Six to eight weeks old female BALB/c mice (n=56)

Comparison

Intramuscular inoculation of recombinant… vs Intramuscular injection of 100 μL inactivated…

Design

Preclinical, randomly divided into seven groups

Follow-up

7 weeks

Authors

YCYimei CaoWenzhou Medical University ZLZengjun LuLanzhou Veterinary Research Institute PSPu SunArmy Medical University

Discussion

Member takes

Implication

Hypothesis-generating for FMDV vaccine platforms; leaves open translation to livestock or humans.

Structured PICO

Population

56 female BALB/c mice, aged 6-8 weeks, were immunized to evaluate the immunogenicity of recombinant pseudotype baculoviruses against foot-and-mouth disease virus over 7 weeks.

Intervention

Intramuscular inoculation of recombinant pseudotype baculoviruses (Bac-GED-P12A3C or Bac-GED-P12A3CT) at 1 × 10^9 or 1 × 10^10 PFU/mouse, with a booster 3 weeks later

Comparator

Intramuscular injection of 100 μL inactivated vaccine, 1 × 10^10 PFU of Bac-GED, or 100 μL of PBS

Outcome

FMDV-specific neutralizing antibodies and gamma interferon (IFN-γ) production at 3 and 7 weekssurrogate

Main Result

Absolute Event Rate: 1917% vs 771%

p-value: p=<0.01

A pseudotype baculovirus expressing FMDV capsid protein and a T-cell immunogen enhanced humoral and cellular immune responses in mice, suggesting a potential new vaccine strategy.

Limitations

Inactivated vaccine produced higher neutralizing antibody titers than the recombinant baculoviruses.
Baculovirus vector itself induced a relatively higher background non-specific IFN-gamma response.

Cite This Study

Cao et al. (2011) studied Foot-and-mouth disease (FMD) vaccination model (n=56). Bac-GED-P12A3CT (recombinant pseudotype baculovirus with T-cell epitopes) vs. Bac-GED-P12A3C (without T-cell epitopes) was evaluated on IFN-gamma production (pg/ml) in splenocytes at 7 weeks (1 × 10^10 PFU dose) (p=<0.01). Recombinant pseudotype baculovirus with T-cell epitopes significantly enhanced IFN-gamma production (P < 0.01) and neutralizing antibody titers (P < 0.05) compared to the recombinant without T-cell epitopes in mice.

synapsesocial.com/papers/6a9450e080af60f92670753f https://doi.org/10.1186/1743-422x-8-77

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A pseudotype baculovirus expressing the capsid protein of foot-and-mouth disease virus and a T-Cell immunogen shows enhanced immunogenicity in mice

Discussion

Member takes

Implication

Structured PICO

Main Result

Limitations

Cite This Study

A pseudotype baculovirus expressing the capsid protein of foot-and-mouth disease virus and a T-Cell immunogen shows enhanced immunogenicity in mice

Discussion

Member takes

Implication

Structured PICO

Main Result

Limitations

Cite This Study

Also Consider

Also Consider