Key result
NMR spectroscopy of a 34 nt influenza virus RNA revealed a double helical structure close to A-form with bulges at A10, A12, and C26, and a Watson-Crick base pair between C11 and G25.
The structural features of the influenza virus A panhandle RNA provide a framework for understanding mutational analysis and RNA-polymerase interactions.
Offers structural template for influenza RNA studies; leaves open utility for polymerase-targeted therapies without clinical validation.
The double-stranded panhandle structure of the influenza virus RNA is important for replication, transcription and packaging into the virion of the virion RNA. The solution structure of a 34 nt RNA which contains the conserved panhandle sequences has been investigated by one- and two-dimensional NMR spectroscopy. The partially complementary 5'- and 3'-ends of the RNA form a double helical structure which is, on average, close to A-form. The stem contains bulges at nucleotides A10, A12 and C26. In between these bulges, C11 and G25 form a Watson-Crick base pair. The structural features of the panhandle provide a framework for the explanation of mutational analysis and for a better understanding of RNA-polymerase interactions.
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Hae‐Kap Cheong (1996) studied Influenza virus A. One- and two-dimensional NMR spectroscopy was evaluated on Secondary structure of the panhandle RNA. NMR spectroscopy of a 34 nt influenza virus RNA revealed a double helical structure close to A-form with bulges at A10, A12, and C26, and a Watson-Crick base pair between C11 and G25.
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