Key result
The in vitro uridylylation of FMDV 3B peptides by the RNA-dependent RNA polymerase 3Dpol requires the 3CD precursor and an internal RNA sequence (cre/bus) containing a critical AAACA motif.
The study identifies the requirements for in vitro uridylylation of FMDV 3B peptides, showing the poly(A) tail is not a significant template.
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Highlights cre/bus motif as antiviral target for FMDV; leaves open in vivo relevance and therapeutic translation.
Nayak et al. (2005) studied Foot-and-Mouth Disease Virus (FMDV) replication. In vitro uridylylation assay components (3Dpol, 3CD, cre/bus RNA, 3B peptides) was evaluated on Uridylylation of FMDV 3B peptides. The in vitro uridylylation of FMDV 3B peptides by the RNA-dependent RNA polymerase 3Dpol requires the 3CD precursor and an internal RNA sequence (cre/bus) containing a critical AAACA motif.
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