January 1, 1995Nucleic Acids ResearchOpen Access

Purine–purine mismatches in RNA helices: evidence for protonated G·A pairs and next-nearest neighbor effects

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Population

12 different RNA duplexes containing A.A, A.G, G.A and G.G mismatches flanked by C-G base pairs

Design

Preclinical

Authors

SMSarah Morse DDDavid E. DraperLabCorp (United States)

Discussion

Member takes

Implication

These parameters should not guide clinical RNA interventions; leaves open incorporation into cardiovascular transcript models.

Structured PICO

Population

12 different RNA duplexes containing A.A, A.G, G.A and G.G mismatches flanked by C-G base pairs

Intervention

UV melting studies under three different buffer conditions (varying salt concentration and pH)

Outcome

Thermodynamic parameters (delta G degrees 37 of denaturation)surrogate

The study provides thermodynamic evidence that purine-purine mismatches affect RNA helix stability and that G.A mismatches can form protonated structures.

Cite This Study

Morse et al. (1995) studied this question.

synapsesocial.com/papers/6a94606d70faba02c63ed823 https://doi.org/10.1093/nar/23.2.302

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Bookmark

Purine–purine mismatches in RNA helices: evidence for protonated G·A pairs and next-nearest neighbor effects

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Purine–purine mismatches in RNA helices: evidence for protonated G·A pairs and next-nearest neighbor effects

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider