What question did this study set out to answer?

Determine whether pre-existing vaccine-induced immune responses against SIV envelope protein drive specific genetic variation in gp120 following viral challenge.

May 1, 1993Journal of General Virology

Sequence Variation in the env Gene of Simian Immunodeficiency Virus Recovered from Immunized Macaques is Predominantly in the V1 Region

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Authors

NANeil AlmondUniversity of Hertfordshire AJAdrian JenkinsPotters Bar Community Hospital AHAlan HeathIziko Museums of South Africa

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Implication

Controlled challenge study demonstrates accelerated V1 region mutation of SIV gp120 in immunized macaques, suggesting immune selection drives viral escape.

Key Points

Determine whether pre-existing vaccine-induced immune responses against SIV envelope protein drive specific genetic variation in gp120 following viral challenge.
Immunized three cynomolgus macaques with recombinant SIV envelope protein preparations and challenged them alongside controls with 10 MID50 of SIVmac251-32H.
Amplified proviral SIV gp120 sequences from peripheral blood at 2 months post-infection using polymerase chain reaction and analyzed V1 through V5 regions.
Sequence diversity across the V2 to V5 regions of gp120 did not distinguish provirus in immunized macaques from unimmunized controls.
Novel V1 region variants recovered from immunized animals diverged significantly from challenge stock and included altered N-linked glycosylation sites, showing significantly greater diversity than variants from control animals (P < 0.001).

Cite This Study

Almond et al. (1993) studied this question.

synapsesocial.com/papers/6a9464496d20e72ae3707ab2 https://doi.org/10.1099/0022-1317-74-5-865

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Implication

Controlled challenge study demonstrates accelerated V1 region mutation of SIV gp120 in immunized macaques, suggesting immune selection drives viral escape.

Sequence Variation in the env Gene of Simian Immunodeficiency Virus Recovered from Immunized Macaques is Predominantly in the V1 Region

Discussion

Member takes

Implication

Key Points

Cite This Study

Sequence Variation in the env Gene of Simian Immunodeficiency Virus Recovered from Immunized Macaques is Predominantly in the V1 Region

Discussion

Member takes

Implication

Key Points

Cite This Study

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