Key result
Chronic atrial fibrillation in patients with mitral valve disease was associated with significantly increased atrial fibrosis and 223 differentially expressed proteins compared to sinus rhythm.
Cross-Sectional (n=12)
No
Absolute Event Rate: 9.54% vs 4.27%
p-value: p=0.003
Label-free proteomic analysis identified 223 differentially expressed proteins associated with atrial fibrosis in patients with chronic atrial fibrillation, providing potential molecular targets for understanding AF mechanisms.
Should not yet change AF management in mitral valve disease; hypothesis-generating for proteomic fibrosis targets.
BACKGROUND: Atrial fibrosis, as a hallmark of atrial structure remodeling, plays an important role in maintenance of chronic atrial fibrillation, but interrelationship of atrial fibrosis and atrial fibrillation is uncertain. Label-free proteomics can implement high throughput screening for finding and analyzing pivotal proteins related to the disease.. Therefore, we used label-free proteomics to explore and analyze differentially proteins in chronic atrial fibrillation patients with mitral valve disease. METHODS: Left and right atrial appendages obtained from patients with mitral valve disease were both in chronic atrial fibrillation (CAF, AF≥6 months, n = 6) and in sinus rhythm (SR, n = 6). One part of the sample was used for histological analysis and fibrosis quantification; other part were analyzed by label-free proteomic combining liquid chromatography with mass spectrometry (LC-MS), we utilized bioinformatics analysis to identify differential proteins. RESULTS: Degree of atrial fibrosis was higher in CAF patients than that of SR patients. 223 differential proteins were detected between two groups. These proteins mainly had vital functions such as cell proliferation, stress response, focal adhesion apoptosis. We evaluated that serine/threonine protein kinase N2 (PKN2), dermatopontin (DP), S100 calcium binding protein B (S100B), protein tyrosine kinase 2 (PTK2) and discoidin domain receptor tyrosine kinase 2 (DDR2) played important roles in fibrotic process related to atrial fibrillation. CONCLUSION: The study presented differential proteins responsible for atrial fibrosis in chronic atrial fibrillation patients through label-free proteomic analysis. We assessed some vital proteins including their characters and roles. These findings may open up new realm for mechanism research of atrial fibrillation.
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Zhang et al. (2013) conducted a cross-sectional in Chronic Atrial Fibrillation with Mitral Valve Disease (n=12). Chronic Atrial Fibrillation vs. Sinus Rhythm was evaluated on Left atrial appendage fibrosis area (%) (p=0.003). Chronic atrial fibrillation in patients with mitral valve disease was associated with significantly increased atrial fibrosis and 223 differentially expressed proteins compared to sinus rhythm.
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