The immobilisation of α‐amylase (E.C.3.2.1.1) on Biozan R with dicyclohexyl carbodiimide as an activator has been studied. The reaction was proved to be influenced by the enzyme support and activator support ratios as well as by the reaction duration. The function correlating the coupled enzyme activity with process parameters was derived by processing the experimental results obtained by means of a second order, composite, centrate, rotatable experimental design, using the multiple regression method. The activity of the coupled enzyme was dependent on the temperature and pH of the incubation reaction. The Michaelis‐Menten constant is higher for the immobilised enzyme as compared with the free one.
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Dumitriu et al. (1985) studied this question.
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