Key result
The integrated cell culture-qRTPCR assay successfully quantified coxsackievirus, echovirus, and poliovirus, achieving three-log microbial inactivation at UV doses of 28-44 mJ/cm2.
ICC-qRTPCR is a practical alternative for the simultaneous quantification of enteroviruses in disinfection studies.
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May streamline enterovirus quantification in lab disinfection studies; leaves open translation to clinical water treatment.
Mayer et al. (2010) studied Enterovirus quantification in disinfection studies. Integrated cell culture-quantitative RTPCR (ICC-qRTPCR) assay vs. Conventional cultural techniques was evaluated on Three-log microbial inactivation at specific UV doses. The integrated cell culture-qRTPCR assay successfully quantified coxsackievirus, echovirus, and poliovirus, achieving three-log microbial inactivation at UV doses of 28-44 mJ/cm2.