Among a number of organisms Pseudomonas fluorescens 6009–2 and mutant Ki–1023 derived from Escherichia coli K–12 by ultraviolet radiation were chosen as suitable strains for the accumulation of l-aspartic acid from fumarate and ammonium salts. By employing these organisms, optimum cultural conditions for the production of l-aspartic acid were investigated. As a result, l-aspartic acid was obtained in a good yield by the advantageous process presented below.
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Kisumi et al. (1960) studied this question.