Key result
A low-volume immunoassay allowed reliable determination of renin in 40 μL plasma with between-run accuracy of -3.3 to +3.0% and precision of 4.9 to 11.3%.
A newly validated low-volume immunoassay enables reliable measurement of direct renin using only 40 μL of plasma, facilitating pharmacodynamic research in pediatric heart failure.
Supports low-volume renin assays for pediatric samples; leaves open clinical adoption pending further validation.
INTRODUCTION: The pharmacotherapy of pediatric patients suffering from heart failure is extrapolated from adults due to missing data in children. OBJECTIVES: Development and validation of a low-volume immunoassay for the reliable determination of renin. EXPERIMENTAL: The immunoassay was validated according to international guidelines. RESULTS: The assay allows the reliable determination of renin in 40 μL plasma within a calibration range of 4-128 pg/mL. Between-run accuracy varied from -3.3 to +3.0% (relative error), while between-run precision ranged from 4.9 to 11.3% (coefficient of variation). CONCLUSION: The low-volume immunoassay facilitates the reliable collection of pharmacodynamic data in children.
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Schaefer et al. (2017) studied Heart failure. Low-volume immunoassay for renin was evaluated on Assay accuracy and precision. A low-volume immunoassay allowed reliable determination of renin in 40 μL plasma with between-run accuracy of -3.3 to +3.0% and precision of 4.9 to 11.3%.
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