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Groups of five Holstein heifers were hysterectonfized on Day 10 of the estrous cycle, and beginning 21 days later were given daily injections of either oxytocin, estradiol, equine luteinizing hormone, adrenocorticotropin, or relaxin. Three additional groups were slaughtered at the end of six, :15, and 30-day control periods. A gradual decrease in luteal tissue weight, progesterone concentration, and 20-fi-ol concentration occurred as the control pe~iod lengthened.
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Brunner et al. (1969) studied this question.
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