What question did this study set out to answer?

To identify the viral envelope glycoproteins that govern host cell tropism and drive membrane fusion in canine distemper virus and measles virus.

March 1, 1995Journal of VirologyOpen Access

The hemagglutinin envelope protein of canine distemper virus (CDV) confers cell tropism as illustrated by CDV and measles virus complementation analysis

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Authors

LSL B SternTel Aviv University MGMichael GreenbergStevens Institute of Technology JGJonathan M. GershoniTel Aviv University

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Implication

In vitro study demonstrates that canine distemper virus hemagglutinin dictates host cell tropism in somatic cell hybrids, indicating that fusion proteins remain functionally interchangeable.

Key Points

To identify the viral envelope glycoproteins that govern host cell tropism and drive membrane fusion in canine distemper virus and measles virus.
Expressed measles virus (MV) and canine distemper virus (CDV) hemagglutinin (HA) and fusion (F) proteins using a vaccinia virus expression system.
Cotransfected homologous and heterologous glycoprotein combinations to assess syncytium formation in permissive cells and screened 12 human-mouse somatic cell hybrids to map susceptibility chromosomes.
Syncytium formation strictly required the coexpression of both F and HA proteins, with heterologous combinations (CDV-F with MV-HA and MV-F with CDV-HA) inducing robust cell fusion.
Two human-mouse somatic hybrids containing human chromosome 19 showed susceptibility to CDV infection and CDV-F plus CDV-HA fusion, while remaining nonsusceptible to MV glycoproteins.
Complementation assays demonstrated that syncytia formed in CDV-sensitive hybrids exclusively in the presence of CDV-HA, confirming HA dictates cell tropism while F remains functionally interchangeable.

Cite This Study

Stern et al. (1995) studied this question.

synapsesocial.com/papers/6a94dabd5426d440e59e5840 https://doi.org/10.1128/jvi.69.3.1661-1668.1995

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Implication

In vitro study demonstrates that canine distemper virus hemagglutinin dictates host cell tropism in somatic cell hybrids, indicating that fusion proteins remain functionally interchangeable.

The hemagglutinin envelope protein of canine distemper virus (CDV) confers cell tropism as illustrated by CDV and measles virus complementation analysis

Discussion

Member takes

Implication

Key Points

Cite This Study

The hemagglutinin envelope protein of canine distemper virus (CDV) confers cell tropism as illustrated by CDV and measles virus complementation analysis

Discussion

Member takes

Implication

Key Points

Cite This Study

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