Studies on cholinesterase. 7. Determination of the molar concentration of pseudo-cholinesterase in serum

Insulin can be separated from protaminle by paper chromatography developing with the upper phase of a mixture of n-butanol, glacial acetic acid and water (3: 1: 4 byvol.).A dye such as bromocresol green is used for locating the zones.2. The concentration ofprotamine and of various proteins in a solution can be estimated on filter paper by retention analysis with a suitable dye, e.g.ery- throsine.The area of the unstained wedge formed above the protein spot is proportional to the amount of protein present.The method can be used to estimate the amount ofprotamine in protamine zinc insulin after removal of insulin by paper chromato- graphy.