Fluorescent products of lipid peroxidation: II. Methods for analysis and characterization

The effects of pH and of the metal chelator, europium (Tric[2,2,6,6‐tetra‐methyl‐3,5‐heptanedionate]), upon fluorescence of lipid peroxidation products were tested, and fluorescence decay times and fluorescence polarization values were determined. Measurements of fluorescence intensity showed that the fluorescence of these compounds was quenched at basic pH and that it was restored by adjustment of pH to neutrality. Metal chelator decreased the fluorescence intensity 8–15%. pH Effects and metal coordination effects are useful for analysis and characterization of these fluorescent products. Fluorescence polarization and fluorescence decay times are also useful analytical techniques for characterization of fluorescent products.