March 5, 2013Skeletal MuscleOpen Access

Canonical Wnt signaling induces BMP-4 to specify slow myofibrogenesis of fetal myoblasts

Q: What is the clinical evidence from this study?

Study design: Other. Population: Skeletal muscle development and fiber type specification. Intervention: Canonical Wnt signaling activation (Wnt-1, Wnt-3a, stabilized β-catenin) vs. Control/Mock. Primary outcome: Myogenic differentiation and slow myofiber specification.

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Key result

Canonical Wnt signaling induces BMP-4 to specify slow myofibrogenesis and inhibit overall myoblast proliferation during fetal myogenesis.

Population

TCF-reporter mice, embryos, muscle groups, fetal myoblasts, C2C12 cells, and primary myoblasts

Comparison

Muscle-specific transgenic expression of… vs Control conditions, or inhibition of BMP-4 by…

Design

Preclinical

Authors

KKKazuki KurodaUniversity of Fukui SKShihuan KuangIndiana University Health MTMakoto M. TaketoKanazawa University

Discussion

Member takes

Implication

Should not inform clinical myopathy management; leaves open Wnt-BMP roles in human fetal myofiber specification.

Structured PICO

Population

Preclinical study using transgenic mice and fetal myoblasts to investigate the role of canonical Wnt signaling in skeletal myogenesis.

Intervention

Muscle-specific transgenic expression of stabilized β-catenin, overexpression of Wnt-1 or Wnt-3a, or treatment with Wnt-3a or BMP-4 recombinant proteins

Comparator

Control conditions, or inhibition of BMP-4 by shRNA or recombinant Noggin protein

Outcome

Myogenic differentiation towards slow muscle phenotype (slow myosin heavy chain expression)surrogate

Canonical Wnt signaling interacts with BMP signaling to induce myogenic differentiation towards a slow muscle phenotype.

Limitations

Embryonic lethality of β-catenin deficient or over-expressing mice precludes some analysis in postnatal muscles without conditional models.

Cite This Study

Kuroda et al. (2013) studied Skeletal muscle development and fiber type specification. Canonical Wnt signaling activation (Wnt-1, Wnt-3a, stabilized β-catenin) vs. Control/Mock was evaluated on Myogenic differentiation and slow myofiber specification. Canonical Wnt signaling induces BMP-4 to specify slow myofibrogenesis and inhibit overall myoblast proliferation during fetal myogenesis.

synapsesocial.com/papers/6a94f4dab1a163ea017683d8 https://doi.org/10.1186/2044-5040-3-5

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Canonical Wnt signaling induces BMP-4 to specify slow myofibrogenesis of fetal myoblasts

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Canonical Wnt signaling induces BMP-4 to specify slow myofibrogenesis of fetal myoblasts

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider