July 1, 2000Journal of Biological ChemistryOpen Access

Inhibition of Spontaneous β2-Adrenergic Activation Rescues β1-Adrenergic Contractile Response in Cardiomyocytes Overexpressing β2-Adrenoceptor

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Population

Transgenic mice with cardiac-specific overexpression of human beta-adrenergic receptor and cultured wild…

Comparison

Pre-incubation with muscarinic receptor agonist… vs No pre-incubation (spontaneous beta-AR signaling)

Design

Preclinical

Authors

SZSheng-Jun ZhangUniversity of Maryland, Baltimore HCHeping ChengElectrophysiology YZYing-Ying ZhouDaqing Oilfield General Hospital

Discussion

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Implication

Constitutive β2-AR activation induces β1-AR desensitization; leaves open whether targeting this crosstalk affects human cardiac contractility.

Structured PICO

Population

Transgenic mice (TG4) with cardiac-specific overexpression of human beta(2)-adrenergic receptor and cultured wild type adult mouse ventricular myocytes with adenovirus-directed overexpression of human beta(2)-AR

Intervention

Pre-incubation with muscarinic receptor agonist carbachol (10^-5 M) or beta(2)-AR inverse agonist ICI 118,551 (5 x 10^-7 M)

Comparator

No pre-incubation (spontaneous beta(2)-AR signaling)

Outcome

beta(1)-AR contractile response and base-line cAMP levelssurrogate

Constitutive beta2-AR activation induces heterologous desensitization of beta1-ARs independent of betaARK1 and Gi proteins, which can be rescued by suppressing constitutive beta2-AR signaling.

Cite This Study

Zhang et al. (2000) studied this question.

synapsesocial.com/papers/6a95004ebb5c3a2606827552 https://doi.org/10.1074/jbc.m909484199

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Implication

Constitutive β2-AR activation induces β1-AR desensitization; leaves open whether targeting this crosstalk affects human cardiac contractility.

Inhibition of Spontaneous β2-Adrenergic Activation Rescues β1-Adrenergic Contractile Response in Cardiomyocytes Overexpressing β2-Adrenoceptor

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Inhibition of Spontaneous β2-Adrenergic Activation Rescues β1-Adrenergic Contractile Response in Cardiomyocytes Overexpressing β2-Adrenoceptor

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider