Key result
Using an optical density cutoff of 0.05, the DiaSorin DNA enzyme immunoassay detected enterovirus RT-PCR products in 35% of cerebrospinal fluid specimens compared to 16% by viral culture.
Why the study?
Does the DiaSorin DNA enzyme immunoassay (DEIA) kit improve detection of enterovirus in cerebrospinal fluid specimens compared to viral culture?
Cross-Sectional (n=198)
No
Does the DiaSorin DNA enzyme immunoassay (DEIA) kit improve detection of enterovirus in cerebrospinal fluid specimens compared to viral culture?
Absolute Event Rate: 35% vs 16%
The DiaSorin DEIA kit is a rapid and sensitive method that can facilitate the implementation of enterovirus RT-PCR in clinical laboratories.
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May increase enterovirus detection in CSF over culture; leaves open prospective validation before routine lab adoption.
Young et al. (2000) conducted a cross-sectional in Suspected viral meningitis (n=198). DiaSorin DNA enzyme immunoassay (DEIA) for enterovirus RT-PCR vs. Viral cell culture was evaluated on Enterovirus detection rate in cerebrospinal fluid specimens. Using an optical density cutoff of 0.05, the DiaSorin DNA enzyme immunoassay detected enterovirus RT-PCR products in 35% of cerebrospinal fluid specimens compared to 16% by viral culture.
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