Rapid diagnosis of enterovirus infection by magnetic bead extraction and polymerase chain reaction detection of enterovirus RNA in clinical specimens

Clinical specimens (cerebrospinal fluid, stool, saliva, blood, pericardial fluid, urine, and cryopreserved or formalin-fixed solid tissue) from patients with acute enteroviral disease, acute cardiac disease, acute myocarditis, and chronic dilated cardiomyopathy

Magnetic bead extraction followed by reverse transcription polymerase chain reaction (RT-PCR) amplification

Cell culture isolation and extraction methods using organic reagents

Sensitivity of enterovirus RNA detectionsurrogate