Why the study?
Does nucleic acid sequence-based amplification improve Enterovirus detection compared to cell culture isolation in clinical specimens?
Does nucleic acid sequence-based amplification improve Enterovirus detection compared to cell culture isolation in clinical specimens?
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The study compares a rapid RNA detection method with traditional cell culture for enterovirus.
Enterovirus (EV) detection by nucleic acid sequence-based amplification was compared with EV isolation in cell culture. The NucliSens Basic kit (bioMerieux) was utilized for RNA detection. For virus isolation, samples were inoculated into MRC-5, primary rhesus monkey kidney, A549, rhabdomyosarcoma, and/or Buffalo green monkey kidney cells.
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Landry et al. (2003) studied this question.
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