Why the study?
Does the Penter RT-PCR test improve the detection of Enterovirus in cerebrospinal fluid specimens of patients with aseptic meningitis compared to cell culture and in-house RT-PCR?
Does the Penter RT-PCR test improve the detection of Enterovirus in cerebrospinal fluid specimens of patients with aseptic meningitis compared to cell culture and in-house RT-PCR?
The new Penter RT-PCR assay provides rapid and sensitive detection of Enterovirus in cerebrospinal fluid, outperforming cell culture and in-house RT-PCR.
May support Penter RT-PCR for Enterovirus detection in CSF; hypothesis-generating and should not yet change practice.
Enterovirus (EV) detection by a new commercially available reverse transcription (RT)-PCR assay (Penter RT-PCR test) was compared with EV isolation from cell cultures and with EV detection by an in-house RT-PCR assay. Of the 54 cerebrospinal fluid specimens collected during a summer outbreak of aseptic meningitis, 52% were positive by cell culture versus 76% by in-house RT-PCR assay and 80% by the new RT-PCR test (52 versus 76 versus 80%; P = 0.003). This new reliable EV RNA detection test is suitable for clinical diagnosis of EV-related meningitis and may improve the management of EV-related neurological syndromes.
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Jacques et al. (2003) studied this question.
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