Key result
Co-expression of rSK1 with rSK2 in HEK 293 cells increased current magnitude by 77% and reduced sensitivity to apamin and UCL 1848 compared to rSK2 alone, demonstrating subunit interaction.
Population
HEK 293 cells
Comparison
Co-expression of rat SK1 (rSK1) and rSK2 genes vs Expression of rSK2 alone
Design
Preclinical
Authors
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No immediate clinical implications from this cell model; leaves open whether heteromeric SK channels form and alter pharmacology in native cardiomyocytes.
Co-expression of rSK1 and rSK2 subunits in HEK 293 cells forms functional heteromeric channels with distinct pharmacological properties compared to homomeric SK2 channels.
Benton et al. (2003) studied this question. Co-expression of rSK1 with rSK2 vs. rSK2 alone was evaluated on Current magnitude and channel pharmacology (sensitivity to block by apamin and UCL 1848). Co-expression of rSK1 with rSK2 in HEK 293 cells increased current magnitude by 77% and reduced sensitivity to apamin and UCL 1848 compared to rSK2 alone, demonstrating subunit interaction.
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