Key result
Ca2+-independent interactions with calmodulin are required for surface expression of SK channels, whereas constitutive association between the two subunits is not essential for gating.
Population
Cells for heterologous expression of SK2 and CaM mutants
Comparison
Expression of SK2 double charge reversal mutant… vs Wild type SK2 and CaM
Design
Preclinical
Authors
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SK2 R464E/K467E abolishes surface expression; leaves open CaM interaction effects on native cardiac SK function.
This study demonstrates that Ca2+-independent interactions with calmodulin are necessary for the surface expression of SK channels, but constitutive association is not required for channel gating.
Lee et al. (2003) studied this question. Mutagenesis of SK2 and CaM vs. Wild type SK2 and CaM was evaluated on Surface expression and channel activity. Ca2+-independent interactions with calmodulin are required for surface expression of SK channels, whereas constitutive association between the two subunits is not essential for gating.
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