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November 1, 1987AJP Renal Physiology

Binding and functional effects of atrial natriuretic factor in isolated rat kidney

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Population

Isolated perfused rat kidneys

Design

Preclinical

Authors

MSMuneya SuzukiCornell UniversityFAFernando Antônio de AlmeidaBrazilian Society of SpeechDNDaniel R. NussenzveigVA North Texas Health Care System

Discussion

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Implication

ANF renal effects at physiological levels merit exploration in cardiorenal models; leaves open human translation from rodent cortex data.

Key Points

  • To examine the distribution and affinity of atrial natriuretic factor binding sites and evaluate how receptor occupancy relates to hemodynamic and excretory responses in isolated perfused rat kidneys.
  • Perfused isolated rat kidneys with 125I-labeled and unlabeled ANF 1-28 across concentrations ranging from 4 pM to 1 microM.
  • Determined binding capacity (Cmax) and apparent affinity (S50) across the cortex, outer medulla, and papilla, while measuring glomerular filtration rate and electrolyte excretion.
  • The cortex contained greater than 90% of total binding sites (Cmax = 6.8 pmol/g tissue; S50 = 54 pM), whereas the papilla contained less than 2% of total sites with lower affinity (S50 = 860 pM).
  • Increases in glomerular filtration rate and fluid and electrolyte excretion were detectable at 10-100 pM and reached maximum levels at 1-10 nM, showing no dissociation between hemodynamic and natriuretic actions below 1 nM.

Structured PICO

P
Population
Isolated perfused rat kidneys
I
Intervention
125I-labeled and unlabeled atrial natriuretic factor (ANF) 1-28 (4 pM to 1 microM)
O
Outcome
Distribution, capacity (Cmax), and apparent affinity (S50) of specific binding of ANF 1-28 in cortex, outer medulla, and papilla, and dose-response curves of the effects of ANF 1-28 on renal hemodynamics and excretion of fluid and electrolytessurrogate

Binding site occupancy in kidney cortex and renal effects of atrial natriuretic factor occur at near physiological concentrations of the hormone.

Cite This Study

Suzuki et al. (1987) studied this question.

synapsesocial.com/papers/6a953b322fdd6c151ff22cd7https://doi.org/10.1152/ajprenal.1987.253.5.f917
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Characterization of receptors for the atrial natriuretic factor in rat renal microvessels1993 · 3 citations
  2. 2Specific membrane receptors for atrial natriuretic factor in renal and vascular tissues.1984 · 262 citations
  3. 3Relationship between renal hemodynamic and natriuretic effects of atrial natriuretic factor1986 · 107 citations
  4. 4Characterization of ANF receptors in cultured renal cortical vascular smooth muscle cells1991 · 10 citations
  5. 5Discrimination and Quantification of Glomerular Receptor Subtypes for Atrial Natriuretic Factor (Anf)1991 · 7 citations