Key result
Reverse transcription droplet digital PCR (RT-ddPCR) was a more sensitive testing method than real-time PCR, which detected norovirus GII in 34.9% of patient and 19.2% of asymptomatic samples.
Why the study?
Quickly identifying and isolating potential contaminants is an effective method to prevent the spread of norovirus outbreaks.
Does RT-ddPCR improve the detection of norovirus GII genotypes in outbreak samples compared to real-time PCR?
Observational (n=376)
Does RT-ddPCR improve the detection of norovirus GII genotypes in outbreak samples compared to real-time PCR?
RT-ddPCR is a more sensitive method than real-time PCR for detecting norovirus in outbreak samples, which may aid in quicker identification and isolation of contaminants.
RT-ddPCR may enhance norovirus GII detection sensitivity; hypothesis-generating for outbreak surveillance and requires prospective validation.
Noroviruses cause acute gastroenteritis with symptoms of diarrhoea and vomiting, and their high infectivity allows outbreaks to readily occur. Quickly identifying and isolating potential contaminants is an effective method to prevent the spread of outbreaks. A total of 376 samples collected from nine outbreaks were categorized as either patient, asymptomatic individual, cook or environmental samples, according to the source of contamination. Using real-time PCR and sequencing analysis, norovirus GII genotypes were detected in 34·9% of samples from patients, 19·2% from asymptomatic individuals, 2·4% from the environment and 1·4% from cooks. Our findings showed contrasting results in samples categories quantified based on the limit of blank and detection limit by reverse transcription droplet digital PCR, which is a more sensitive testing method than real-time-PCR.
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Song et al. (2022) conducted an observational in Norovirus outbreak (n=376). Reverse transcription droplet digital PCR (RT-ddPCR) vs. Real-time PCR was evaluated on Detection of norovirus GII genotypes. Reverse transcription droplet digital PCR (RT-ddPCR) was a more sensitive testing method than real-time PCR, which detected norovirus GII in 34.9% of patient and 19.2% of asymptomatic samples.
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