April 1, 2006Journal of Clinical MicrobiologyOpen Access

Use of TaqMan Real-Time Reverse Transcription-PCR for Rapid Detection, Quantification, and Typing of Norovirus

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Why the study?

Does TaqMan real-time RT-PCR improve the rapid detection and quantification of Norovirus compared to conventional RT-PCR in clinical and water samples?

Population

92 clinical stool samples and 33 water samples from confirmed NoV outbreaks and suspected NoV contamination…

Comparison

TaqMan real-time reverse transcription-PCR… vs Conventional RT-PCR assay.

Authors

ATA. Angelica TrujilloCenters for Disease Control and Prevention KMKaren A. McCaustlandCenters for Disease Control and Prevention DZDu‐Ping ZhengCenters for Disease Control and Prevention

Discussion

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Implication

May enhance Norovirus detection sensitivity in clinical and water samples; leaves open prospective validation before routine adoption.

Structured PICO

Does TaqMan real-time RT-PCR improve the rapid detection and quantification of Norovirus compared to conventional RT-PCR in clinical and water samples?

Population

92 clinical stool samples (65 NoV-positive, 27 NoV-negative) and 33 water samples from confirmed NoV outbreaks and suspected NoV contamination cases.

Intervention

TaqMan real-time reverse transcription (RT)-PCR assays for Norovirus strains GI, GII, and GIV.

Comparator

Conventional RT-PCR assay.

Outcome

Detection and quantification of Norovirus RNA.surrogate

TaqMan real-time RT-PCR is a highly sensitive and specific method for rapid detection and quantification of Norovirus GI, GII, and GIV in both clinical and environmental samples.

Limitations

GIV stool standard had a lower detection limit possibly due to nucleases and other RT/PCR inhibitors in stool.

Cite This Study

Trujillo et al. (2006) studied this question.

synapsesocial.com/papers/6a729488ac440176ef2abed9 https://doi.org/10.1128/jcm.44.4.1405-1412.2006

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Use of TaqMan Real-Time Reverse Transcription-PCR for Rapid Detection, Quantification, and Typing of Norovirus

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Use of TaqMan Real-Time Reverse Transcription-PCR for Rapid Detection, Quantification, and Typing of Norovirus

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

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