The Activity of Bile Salts and Certain Detergents on the Hepatic Storage and Protein‐binding of Sulphobromophthalein

Bile salts and certain detergents interfered with the uptake of sulphobromophthalein (BSP) by the liver from the blood. One variety of India ink (but not another) could release BSP which had already been stored in the liver. It was shown that bile salts, alkaline shellac (the active ingredient of the effective India ink) and various anionic detergents interfered with the bond between protein and BSP in vitro as well as with the hepatic storage of BSP in vivo. It is suggested that the storage of BSP is largely, if not entirely, by means of binding to hepatic protein.