We developed a transformation system for Aspergillus oryzae using the Aspergillus nidulans sC gene encoding ATP sulfurylase as a selectable marker. The sC− mutants can be readily isolated by positive selection for selenate resistance, thereby the niaD− mutant strain of A. oryzae was bestowed with the sC− mutation. Transformation of the A. oryzae host (niaD−, sC−) with the plasmid carrying A. nidulans sC gave random and multi-copy integrants, while that with the A. oryzae niaD-carrying plasmid occurred mainly by single-copy and homologous integration events (more than 50% frequency), indicating that with this transformation system, the transformation marker could be selected according to the integration pattern one desires.
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Yamada et al. (1997) studied this question.