Key result
In isolated rat ventricular myocytes, hypoxia in the absence of glucose induced progressive electromechanical failure and ATP depletion, with irreversible damage occurring after 4 hours of hypoxia.
Population
Isolated rat ventricular myocytes in monolayer culture
Comparison
Hypoxia and reoxygenation in the presence and… vs Normoxia or hypoxia in the presence of glucose
Design
Preclinical
Follow-up
Up to 4 hours
Authors
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Does not warrant clinical changes in ischemia; hypothesis-generating for myocyte viability thresholds in translational models.
In isolated rat ventricular myocytes, the duration of hypoxia without exogenous substrate determines the shift from reversible to irreversible cellular damage, marked by ATP depletion and LDH release.
Fantini et al. (1990) studied Isolated rat ventricular myocytes. Hypoxia and reoxygenation in the presence and absence of glucose vs. Basal properties / presence of glucose was evaluated on Action potentials, contractions, and biochemical parameters (ATP, lactate, LDH). In isolated rat ventricular myocytes, hypoxia in the absence of glucose induced progressive electromechanical failure and ATP depletion, with irreversible damage occurring after 4 hours of hypoxia.
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