February 1, 1991Circulation

Mechanisms of reoxygenation injury in cultured ventricular myocytes.

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Population

Cultured chick ventricular myocytes

Comparison

Severe hypoxia for up to 3 hours followed by… vs Hypoxia alone, or reoxygenation without…

Design

Preclinical

Follow-up

Up to 3 hours of hypoxia plus reoxygenation

Authors

RQRobert A. QuaifeCardiac Imaging OKOsami KohmotoThe University of Tokyo WBWilliam H. BarryBibliothèque Nationale de France

Discussion

Member takes

Implication

Hypothesis-generating for hypercontracture and radical-mediated reoxygenation injury; leaves open clinical translation and therapeutic targeting.

Structured PICO

Population

Cultured chick ventricular myocytes

Intervention

Severe hypoxia for up to 3 hours (in 20 mM 2-deoxyglucose, zero glucose, and 5 mM pyruvate) followed by reoxygenation, with or without pretreatment with 2,3-butanedione monoxime, cyanide, or superoxide dismutase and catalase

Comparator

Hypoxia alone, or reoxygenation without pretreatment

Outcome

Lactate dehydrogenase (LDH) release, intracellular calcium concentration ([Ca2+]i), and cell motion/hypercontracturesurrogate

Reoxygenation injury in ventricular myocytes appears to be driven by calcium-dependent hypercontracture and oxygen free radicals rather than an abrupt increase in intracellular calcium during reoxygenation.

Cite This Study

Quaife et al. (1991) studied this question.

synapsesocial.com/papers/6a826b7afe5ce613f33acfd4 https://doi.org/10.1161/01.cir.83.2.566

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Implication

Hypothesis-generating for hypercontracture and radical-mediated reoxygenation injury; leaves open clinical translation and therapeutic targeting.

Mechanisms of reoxygenation injury in cultured ventricular myocytes.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Mechanisms of reoxygenation injury in cultured ventricular myocytes.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider