Key result
A serum PRV whole-virus indirect IgG ELISA adapted to swine oral fluid showed good binary diagnostic performance (AUC = 93%) and excellent assay repeatability (ICC = 99.3%).
Why the study?
The study evaluated the detection of pseudorabies virus antibodies in swine oral fluid.
Does a serum PRV whole-virus indirect IgG ELISA adapted to oral fluid detect PRV antibodies in swine?
Does a serum PRV whole-virus indirect IgG ELISA adapted to oral fluid detect PRV antibodies in swine?
Effect estimate: AUC 93%
A serum PRV whole-virus indirect IgG ELISA adapted to oral fluid demonstrates good diagnostic performance for detecting PRV antibodies in swine, suggesting its potential utility in pseudorabies surveillance.
May facilitate non-invasive PRV monitoring in swine; extends serum ELISA to oral fluid but remains hypothesis-generating.
We evaluated the detection of pseudorabies virus (PRV) antibodies in swine oral fluid. Oral fluid and serum samples were obtained from 40 pigs allocated to 4 treatment groups (10 pigs/group): negative control (NC); wild-type PRV inoculation (PRV 3CR Ossabaw; hereafter PRV); PRV vaccination (Ingelvac Aujeszky MLV; Boehringer Ingelheim; hereafter MLV); and PRV vaccination followed by PRV inoculation at 21 d post-vaccination (MLV-PRV). Using a serum PRV whole-virus indirect IgG ELISA (Idexx Laboratories) adapted to the oral fluid matrix, PRV antibody was detected in oral fluid samples from treatment groups PRV, MLV, and MLV-PRV in a pattern similar to serum. Vaccination alone produced a low oral fluid antibody response (groups MLV and MLV-PRV), but a strong anamnestic response was observed following challenge with wild-type virus (group PRV). Analyses of the oral fluid PRV indirect IgG ELISA results showed good binary diagnostic performance (area under ROC curve = 93%) and excellent assay repeatability (intra-class correlation coefficient = 99.3%). The demonstrable presence of PRV antibodies in swine oral fluids suggests the possible use of oral fluids in pseudorabies surveillance.
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Cheng et al. (2020) studied Pseudorabies virus (PRV) (n=40). Serum PRV whole-virus indirect IgG ELISA adapted to oral fluid vs. Negative control was evaluated on Binary diagnostic performance (area under ROC curve) (AUC 93%). A serum PRV whole-virus indirect IgG ELISA adapted to swine oral fluid showed good binary diagnostic performance (AUC = 93%) and excellent assay repeatability (ICC = 99.3%).
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