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The biodegradability of aniline was evaluated under two different anaerobic conditions, denitrifying and methano-genic. In addition, under denitrifying conditions, the influence of bicarbonate was studied. Anaerobic sewage digester sludge and estuarine sediment were used as heterogeneous sources of bacteria. Under anaerobic denitrifying conditions amended with bicarbonate, aniline was completely mineralized to CO2 and N2. After an initial lag period, N2 recoveries of 74 and 100% were obtained for sludge and sediment cultures, respectively. Under anaerobic denitrifying conditions with no bicarbonate, aniline depletion was observed; however, stoichiometric quantities of N2 were not produced from mineralization and were in fact inhibited below background controls. Under methanogenic conditions, aniline concentrations remained unchanged for >31 weeks. A metabolite of aniline, 4-hydroxybenzoate, was detected in bicarbonate-amended denitrifying cultures.
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De et al. (1994) studied this question.
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