Key result
Proteomic profiling identified 69 common discrepant transcription factors, with RBL1 (P107) and TBX20 emerging as key candidates involved in the loss of neonatal mouse cardiac regeneration capacity.
Why the study?
The key transcription factors related to the loss of cardiac regeneration capacity in neonatal mice remain largely unknown.
RBL1 (P107) and TBX20 were identified as key transcription factor candidates involved in the loss of cardiac regeneration capacity in neonatal mice.
Answers are grounded in this paper and cite their sources.
RBL1 and TBX20 may regulate neonatal cardiac regeneration loss in mice; hypothesis-generating, requires validation before any clinical consideration.
The heart lacks complete regeneration capacity. In mice, the cardiac apex can regenerate 1 day after birth, although 7 days after birth the repair occurs with a fibrous scar. However, the key transcription factors (TFs) related to this loss of regeneration capacity remain largely unknown. We aimed to find candidates for key TFs using proteomic profiling and comparison during loss of neonatal mouse cardiac regeneration capacity, with preliminary validation using western blotting (WB) and real-time quantitative polymerase chain reaction (RT-qPCR). A total of 69 common discrepant TFs with similar variation trends in two TF response element experiments were identified, 18 of which were matched to known key signaling pathways of cardiac regeneration after pathway enrichment of downstream genes. Validation using RT-qPCR-selected DACH1, RBL1 (P107), and TBX20, and further validation with WB-selected RBL1 (P107) and TBX20. We therefore identified two candidates for key TFs in the loss of mouse cardiac apex regeneration capacity. TBX20 has been biologically validated, and RBL1 (P107) needs to be validated in the future.
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Wang et al. (2019) studied Neonatal mouse cardiac regeneration capacity loss. Proteomic profiling was evaluated on Identification of key transcription factors. Proteomic profiling identified 69 common discrepant transcription factors, with RBL1 (P107) and TBX20 emerging as key candidates involved in the loss of neonatal mouse cardiac regeneration capacity.
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