Key result
Transgenic mice enabling conditional Cre/loxP-mediated expression of human CAR facilitated specific and efficient adenoviral transduction of primary immune cell populations in vitro.
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The development of a transgenic mouse model expressing human CAR enables efficient adenoviral transduction of primary murine immune cells, overcoming previous limitations in gene delivery.
Ectopic gene expression studies in primary immune cells have been notoriously difficult to perform due to the limitations in conventional transfection and viral transduction methods. Although replication-defective adenoviruses provide an attractive alternative for gene delivery, their use has been hampered by the limited susceptibility of murine leukocytes to adenoviral infection, due to insufficient expression of the human coxsackie/adenovirus receptor (CAR). In this issue of the European Journal of Immunology, Heger et al. [Eur. J. Immunol. 2015. 45: XXXX-XXXX] report the generation of transgenic mice that enable conditional Cre/loxP-mediated expression of human CAR. The authors demonstrate that this R26/CAG-CAR∆1(StopF) mouse strain facilitates the faithful monitoring of Cre activity in situ as well as the specific and efficient adenoviral transduction of primary immune cell populations in vitro. Further tweaking of the system towards more efficient gene transfer in vivo remains a future challenge.
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Clausen et al. (2015) conducted an editorial in Gene delivery into primary immune cells. Transgenic mice expressing human CAR (R26/CAG-CAR∆1(StopF)) was evaluated on Adenoviral transduction of primary immune cell populations in vitro. Transgenic mice enabling conditional Cre/loxP-mediated expression of human CAR facilitated specific and efficient adenoviral transduction of primary immune cell populations in vitro.
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