Key result
Cardiac fibroblasts from adult rats induced significant collagen gel contraction to 47% of the initial area after 2 days in serum-free medium.
This in vitro model demonstrates that adult rat cardiac fibroblasts can contract collagen gels even in serum-free conditions, providing a baseline for studying cardiac fibrosis and remodeling.
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Provides serum-free baseline for fibroblast contraction models; leaves open translation to human cardiac fibrosis or clinical relevance.
The aim of the present study was to investigate whether collagen gel contraction can be induced by cardiac fibroblasts in serum-free conditions. Cardiac fibroblasts (from normal male adult rats) from passage 2 were cultured to confluency and added to a hydrated collagen gel in Dulbecco's Modified Eagle's Medium with or without fetal bovine serum for 1, 2, 3 or 7 days. Control gels containing adult rat cardiac fibroblasts showed a significant amount of contraction after 2 days of incubation in a serum-free medium, causing a contraction to 47% of the area at the start of the incubation. Increasing the percent of fetal bovine serum induced further stimulation of the collagen gel contraction by cardiac fibroblasts. Optimal conditions for collagen gel contraction by adult fibroblasts were obtained with 100,000 cells incubated for 1 (up to 3) days. The presence of insulin-transferrin-selenium in the medium caused a more pronounced stimulation of the collagen gel contraction by cardiac fibroblasts, while sodium azide inhibited this contraction.
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Lijnen et al. (2001) studied this question. Serum-free medium vs. Fetal bovine serum was evaluated on Collagen gel contraction area. Cardiac fibroblasts from adult rats induced significant collagen gel contraction to 47% of the initial area after 2 days in serum-free medium.
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