Key result
Telmisartan completely blocked angiotensin II-induced collagen contraction by adult rat cardiac fibroblasts, demonstrating the effect is mediated by the angiotensin II type 1 receptor.
Why the study?
Does telmisartan prevent angiotensin II-induced collagen gel contraction in adult rat cardiac fibroblasts?
Does telmisartan prevent angiotensin II-induced collagen gel contraction in adult rat cardiac fibroblasts?
Angiotensin II-induced collagen gel contraction by adult rat cardiac fibroblasts is mediated by the angiotensin II type 1 receptor, as it is completely blocked by telmisartan.
Answers are grounded in this paper and cite their sources.
May inform ARB mechanisms in fibroblast remodeling; leaves open translation to human cardiac fibrosis.
The possible contributions of the angiotensin receptor subtypes 1 and 2 on the angiotensin II-induced collagen gel contraction by adult rat cardiac fibroblasts were studied using the specific angiotensin receptor type 1 and 2 antagonists telmisartan and P-186, respectively. Cardiac fibroblasts (from normal male adult rats) from passage 2 were cultured to confluency and added to a hydrated collagen gel, with or without angiotensin II, angiotensin II plus telmisartan, or angiotensin II plus P-186 in Dulbecco's Modified Eagle's Medium containing 5% fetal bovine serum for 1, 2, or 3 days. Control gels containing adult rat cardiac fibroblasts showed a significant amount of contraction after 3 days of incubation, causing a contraction to 67.9 +/- 7.1% of the area after 1 day. Angiotensin II (10(-7) M) stimulated (p < or = 0.05) the contraction of collagen mediated by cardiac fibroblasts after 1, 2, or 3 days. Telmisartan (10(-7) M) completely blocked the angiotensin II-induced collagen contraction by cardiac fibroblasts. P-186 (10(-7) M) had no effect on the angiotensin II-induced collagen contraction by cardiac fibroblasts. Addition of telmisartan and P-186 alone did not affect the collagen gel contraction by cardiac fibroblasts. Our data demonstrate that the effects of angiotensin II on the collagen gel contraction by adult rat cardiac fibroblasts are angiotensin II type 1 receptor mediated because they were abolished by the specific angiotensin II type 1 receptor antagonist telmisartan but not by the specific angiotensin II type 2 receptor antagonist P-186.
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Lijnen et al. (2001) studied Angiotensin II-induced collagen gel contraction. Telmisartan and P-186 vs. Angiotensin II alone and control gels was evaluated on Collagen gel contraction (p=< or = 0.05). Telmisartan completely blocked angiotensin II-induced collagen contraction by adult rat cardiac fibroblasts, demonstrating the effect is mediated by the angiotensin II type 1 receptor.
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