Assessing recoveries of branched-chain volatile fatty acids or amino acids in dual-flow continuous cultures limited in rumen-degraded protein without or with supplemental leucine

, low RDP increased de novo synthesis of most amino acids from cellulose, whereas de novo synthesis of leucine increased from starch more than from cellulose. Results are discussed to further document the relative sinks of BCAA and BCVFA in bacterial cell constituents, documenting their importance in the rumen.