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Twenty ejaculates of semen from 17 dairy bulls were extended to contain 200 10 and 20 106 sperm per milliliter in yolk citrate and whole milk extenders and frozen with 7% glycerol. The semen frozen with 200 106 sperm per milliliter was re-extended, after thawing, to 20 106 sperm per milliliter in yolk citrate, whole milk, skimmilk, and CUE, and the motility of these semen samples was estimated during 72 hr of storage at 5 C.
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Desjardins et al. (1962) studied this question.
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