Key result
Suppression of ribosomal stalk proteins P1 and P2 by RNAi resulted in an approximately three-fold increase in FMDV IRES-dependent translation compared to control cells.
Effect estimate: about three fold higher
The study highlights a functional connection between the ribosomal stalk and picornavirus IRES-dependent translation.
Ribosomal stalk proteins may regulate picornavirus IRES translation; leaves open whether P1/P2 targeting affects viral replication in vivo.
A human cell line, in which expression of the ribosomal stalk proteins P1 and P2 has been suppressed by RNAi technology, has been used to test how the loss of these proteins affects IRES-dependent translation. Foot-and-mouth disease virus (FMDV) IRES-dependent translation from a bicistronic construct is about three fold higher in the P1/P2-depleted cells than in control cells in the presence of Lb protease. By contrast, no effect on Hepatitis C virus (HCV) IRES translation was observed. These results emphasize the functional heterogeneity of the IRES and they highlight a functional connection between the ribosomal stalk and picornavirus IRES-dependent translation.
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Martínez‐Azorín et al. (2008) studied this question. Suppression of ribosomal stalk proteins P1 and P2 by RNAi vs. Control cells was evaluated on FMDV IRES-dependent translation (about three fold higher). Suppression of ribosomal stalk proteins P1 and P2 by RNAi resulted in an approximately three-fold increase in FMDV IRES-dependent translation compared to control cells.
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