Key result
The new optical sensor successfully detected the presence of proteins in 100% of cases within a focus group of 16 patients with low-level proteinuria, whereas standard urine test strips yielded 94% false-negative results.
Why the study?
Does a new UV optical absorption method improve the detection of total proteinuria compared to standard urine test strips in patients with CKD, diabetes, or hypertension?
Observational (n=261)
No
Does a new UV optical absorption method improve the detection of total proteinuria compared to standard urine test strips in patients with CKD, diabetes, or hypertension?
Absolute Event Rate: 100% vs 6.25%
A novel UV optical absorption method successfully detects total proteinuria in patients where standard urine test strips yield false negative results, highlighting a specific focus group with normal albumin but elevated total protein.
May improve low-level proteinuria detection over strips; leaves open validation in larger prospective cohorts.
The detection of small quantities of proteinuria has gained significance as multiple studies have demonstrated its diagnostic, pathogenic, and prognostic importance. More than 260 samples of urine taken from the patients suffering chronic kidney disease (CKD), diabetes and hypertension have been analysed in the certified laboratory, with urine analyser H-50 (urine test strips) and with an optoelectronic set-up specially designed for this study. Albumin, protein and creatinine concentrations have been determined in the laboratory and the data thoroughly analysed with the aim to find new approaches to tackle the lowered level proteinuria problems. Special attention has been paid to a particular screening focus group of 16 patients all having normal or slightly abnormal levels of albumin in parallel with enhanced levels of total protein (45% cases) up to 0.4 g/L. A fair correlation between the maxima in the protein, protein/creatinine, protein/albumin values and CKD in the focus group has been observed. The urine test strips method gave 94% negative false results for the focus group whereas the new sensor has shown in all cases the presence of proteins. The sensor signals higher than the mean in this focus group were obtained for the donors with the diagnosed CKD and some other diseases. The new method is based on the optical absorption measurements (285 nm) in the protein fractions received with use of the commercial desalting columns PD-10. The method can be applied in the wide region of protein concentrations from ≤0.1 g/L up to the levels of severe proteinuria (~10g/L).
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Sünter et al. (2015) conducted an observational in Chronic kidney disease, diabetes, and hypertension (n=261). Optical absorption sensor (285 nm) after urine fractionation vs. Urine test strips (H-50) and automated laboratory assays was evaluated on Detection of proteinuria in a focus group of 16 patients with low-level albuminuria but enhanced total protein. The new optical sensor successfully detected the presence of proteins in 100% of cases within a focus group of 16 patients with low-level proteinuria, whereas standard urine test strips yielded 94% false-negative results.
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