November 7, 2000Circulation
Functional Living Trileaflet Heart Valves Grown In Vitro
Key result
Autologous tissue-engineered heart valves grown in vitro functioned up to 5 months in lambs, resembling normal heart valves in microstructure, mechanical properties, and extracellular matrix formation.
Why the study?
Does implantation of autologous tissue-engineered heart valves grown in a biomimetic flow culture system result in functional and structurally mature valves in a lamb model?
Population
6 lambs (weight 19±2.8 kg) undergoing native pulmonary leaflet resection
Design
Preclinical
Follow-up
up to 20 weeks
Authors
Discussion
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Member takes
Implication
Supports preclinical valve engineering; leaves open human translation pending clinical trials.
Structured PICO
Does implantation of autologous tissue-engineered heart valves grown in a biomimetic flow culture system result in functional and structurally mature valves in a lamb model?
P
Population
6 lambs (weight 19±2.8 kg) implanted with autologous tissue-engineered pulmonary valves and followed for up to 20 weeks.
I
Intervention
Implantation of autologous tissue-engineered trileaflet heart valves (fabricated from bioabsorbable polymers and seeded with autologous ovine myofibroblasts and endothelial cells, grown for 14 days in a pulse duplicator in vitro system)
O
Outcome
Valve function and structural integrity (assessed by echocardiography, histology, mechanical properties, and extracellular matrix content) up to 20 weekssurrogate
Autologous tissue-engineered heart valves grown in a biomimetic flow culture system can function up to 5 months in vivo with structural and mechanical properties resembling native valves.
Cite This Study
Hoerstrup et al. (2000) studied Pulmonary valve replacement (animal model) (n=6). Autologous tissue-engineered trileaflet heart valves was evaluated on Valve function, microstructure, and mechanical properties. Autologous tissue-engineered heart valves grown in vitro functioned up to 5 months in lambs, resembling normal heart valves in microstructure, mechanical properties, and extracellular matrix formation.