Characterization of Bio-Absorbable and Biomimetic Granules Produced From Animal Bone by the High Velocity Rotation-Crushing and Demineralizing Technique

Bio-absorbable granules originated from animal bone were easily prepared by using high velocity rotation- crushing apparatuses. Frozen bovine bone, porcine bone and human femoral bone were crushed together with saline ice for 1 min under 12,000 rpm-rotation number of a stainless-blade in a stainless vessel or a ZrO2-blade in a ZrO2 vessel. The crushed granules exhibited granular sizes of 0.5-2 mm, which were efficient for regeneration of alveolar bone. They were dissolved at 293K under 500 rpm for 20 min in 2.0% HNO3 aqueous solutions to obtain completely demineralized bone matrix (DBM) granules. The DBM granules were implanted into the subcutaneous tissue of the back region in nude mice. At 4-6 weeks after the implantation, osteoinduction or cartilage-induction as well as bio-absorption were recognized on the surface layers of both DBM granules. The DBM granules may be applied as new biomimetic materials for bone-regeneration therapy and physiological activation substances for biochemistry.