Extrachromosomal DNA was isolated from germinated mung bean as well as from its callus culture. Methods followed for the isolation of cccDNA gave a heterogeneous mixture of DNA from 4.2 kb to 140 bp in size. Hybridization with a repetitive DNA probe from mung bean chromosomal DNA showed extensive dispersion, similar in chromosomal and extrachromosomal locations. An electron micrograph of purified extrachromosomal DNA revealed some circular DNA molecules with hanging tails.
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Bhattacharyya et al. (1986) studied this question.
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