April 5, 2017AJP Cell Physiology

Balance between S -nitrosylation and denitrosylation modulates myoblast proliferation independently of soluble guanylyl cyclase activation

Q: What is the clinical evidence from this study?

Population: Myogenesis. Intervention: GSNOR inhibition (GSNORi or siRNA) and CysNO supplementation vs. Control (absence of inhibitors/supplementation). Primary outcome: Myoblast proliferation and fusion index.

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Key result

Inhibiting GSNOR or supplementing with CysNO increased intracellular RSNO and enhanced myoblast proliferation while decreasing fusion, independently of cGMP signaling.

Population

Primary cultures of chick embryonic skeletal muscle cells

Comparison

S-nitrosocysteine, 8-Br-cGMP, DETA-NO, or… vs Control cultures without the specific…

Design

Preclinical

Follow-up

72 h

Authors

AYAline YamashitaUniversity of Minnesota MAMaryana T. C. Ancillotti LRLuciana P. RangelUniversidade Federal do Rio de Janeiro

Discussion

Member takes

Implication

Cautions against clinical use of GSNOR inhibition; leaves open its role in cardiac muscle regeneration.

Structured PICO

Population

In vitro study using primary cultures of chick embryonic skeletal muscle cells to investigate myoblast proliferation and fusion.

Intervention

S-nitrosocysteine (CysNO), 8-Br-cGMP, DETA-NO, or inhibitors for NO synthase (NOS), GSNOR (GSNORi or siRNA), soluble guanylyl cyclase (sGC), or a combination of these

Comparator

Control cultures without the specific supplements or inhibitors

Outcome

Myoblast proliferation, myoblast fusion index, GSNOR activity, protein expression, RSNO, cGMP, and cell morphologysurrogate

Myoblast proliferation is enhanced by increasing RSNO and regulated by GSNOR activity independently of cGMP production and signaling.

Cite This Study

Yamashita et al. (2017) studied Myogenesis. GSNOR inhibition (GSNORi or siRNA) and CysNO supplementation vs. Control (absence of inhibitors/supplementation) was evaluated on Myoblast proliferation and fusion index. Inhibiting GSNOR or supplementing with CysNO increased intracellular RSNO and enhanced myoblast proliferation while decreasing fusion, independently of cGMP signaling.

synapsesocial.com/papers/6a95c113471b35fa7b54fdac https://doi.org/10.1152/ajpcell.00140.2016

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Balance between S -nitrosylation and denitrosylation modulates myoblast proliferation independently of soluble guanylyl cyclase activation

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Balance between S -nitrosylation and denitrosylation modulates myoblast proliferation independently of soluble guanylyl cyclase activation

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider