What question did this study set out to answer?

To identify and characterize novel genes in Saccharomyces cerevisiae that require the SSD1-v allele for viability and to determine their roles in cell morphogenesis.

November 1, 1995GeneticsOpen Access

LAS1 is an essential nuclear protein involved in cell morphogenesis and cell surface growth.

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To identify and characterize novel genes in Saccharomyces cerevisiae that require the SSD1-v allele for viability and to determine their roles in cell morphogenesis.
Screened and isolated S. cerevisiae mutants dependent on SSD1-v for viability, identifying the novel gene LAS1 alongside SIT4, BCK1/SLK1, and SMP3.
Assessed cell cycle arrest, cellular morphology, and the localization of actin and Spa2 under conditions of LAS1 loss-of-function and LAS1 overexpression.
Loss of essential LAS1 function arrests cells as 80% unbudded cells and 20% large-budded cells with an accumulation of vesicles at the mother-daughter neck.
Overexpression of LAS1 induces extra cell surface projections, disrupts actin and Spa2 localization, and triggers peripheral accumulation of electron-dense structures.

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Implication

Genetic analysis reveals that LAS1 is essential for bud formation and surface growth in Saccharomyces cerevisiae, suggesting a nuclear role in regulating morphogenesis.

Cite This Study

Doseff et al. (1995) studied this question.

synapsesocial.com/papers/6a95c366f3e79ccb8a319547 https://doi.org/10.1093/genetics/141.3.857

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Implication

Genetic analysis reveals that LAS1 is essential for bud formation and surface growth in Saccharomyces cerevisiae, suggesting a nuclear role in regulating morphogenesis.

LAS1 is an essential nuclear protein involved in cell morphogenesis and cell surface growth.

Key Points

Discussion

Implication

Cite This Study

LAS1 is an essential nuclear protein involved in cell morphogenesis and cell surface growth.

Key Points

Discussion

Implication

Cite This Study

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