Long Non-coding RNA Expression Profile and Functional Analysis in Children With Acute Fulminant Myocarditis

Figures

• 

  Figure 1. Expression profile of lncRNAs and mRNAs in AFM patients. Volcano plots were used to distinguish differentially expressed lncRNAs (A) and mRNAs (B). Hierarchical clustering analysis of differentially expressed lncRNAs (C) and mRNAs (D). Distribution of dysregulated lncRNAs in human chromosomes (E). Red and blue represent upregulated and downregulated expression, respectively. M indicates the experimental group; N indicates the normal group.

• 

  Figure 2. Comparison of the relative RNA expression levels between AFM patients and normal children. The expression of 2 upregulated mRNAs (A) and 3 downregulated and 1 upregulated lncRNA (B) were validated using the 2−ΔΔCt method. The data are displayed as the mean ± SD, and samples of the two groups were compared using the Mann-Whitney U test. *P < 0.05; AFM acute fulminant myocarditis; lncRNAs long non-coding RNAs.

• 

  Figure 3. The top 30 GO terms for cis- (A) or trans-acting lncRNAs (D). The top 10 KEGG pathways for cis- (B) or trans-acting lncRNAs (C). BP biological process; CC cellular component; MP molecular function.

• 

  Figure 4. lncRNA-mRNA network analysis of the T cell receptor and Jak-STAT signaling pathways (Pearson's coefficient > 0.95). Red ovals represent mRNAs, blue rectangles represent lncRNAs, a line represents the correlation.

• 

  Figure 5. Cis/trans lncRNA target prediction in the T cell receptor and Jak-STAT signaling pathways. Red ovals represent mRNAs, blue rectangles represent lncRNAs. The solid line indicates a trans lncRNA, and the dotted line indicates a cis lncRNA.