What question did this study set out to answer?

To identify and localize specific carrageenan types within the cell walls and intercellular matrix across different life cycle stages of Chondrus crispus.

December 1, 1975Phycologia

Carrageenans in the cell walls of Chondrus crispus Stack. (Rhodophyceae, Gigartinales). I. Localization with fluorescent antibody

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Authors

EGElizabeth Gordon-MillsFamily Court of Australia EMEsther L. McCandlessMcMaster University

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Implication

Immunofluorescence analysis demonstrates stage-specific carrageenan localization in Chondrus crispus, indicating that initial cells synthesize the characteristic carrageenan of each generation.

Key Points

To identify and localize specific carrageenan types within the cell walls and intercellular matrix across different life cycle stages of Chondrus crispus.
Applied the fluorescent antibody technique using specific antibodies raised against κ-carrageenan and γ-carrageenan on tissue sections.
Assessed differential antibody staining across vegetative female gametophytes, vegetative sporophytes, carposporophyte tissue, and tetraspores.
Anti-κ-carrageenan antibodies stained vegetative cell walls of the female gametophyte and cross walls of tetraspores, while showing no staining above control levels in vegetative sporophyte or carposporophyte tissue.
Anti-γ-carrageenan antibodies stained vegetative sporophyte sections and carposporophyte cell walls, but did not stain female gametophytes or tetraspore cross walls.

Cite This Study

Gordon-Mills et al. (1975) studied this question.

synapsesocial.com/papers/6a961ff69769044d8735904e https://doi.org/10.2216/i0031-8884-14-4-275.1

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Implication

Immunofluorescence analysis demonstrates stage-specific carrageenan localization in Chondrus crispus, indicating that initial cells synthesize the characteristic carrageenan of each generation.

Carrageenans in the cell walls of Chondrus crispus Stack. (Rhodophyceae, Gigartinales). I. Localization with fluorescent antibody

Discussion

Member takes

Implication

Key Points

Cite This Study

Carrageenans in the cell walls of Chondrus crispus Stack. (Rhodophyceae, Gigartinales). I. Localization with fluorescent antibody

Discussion

Member takes

Implication

Key Points

Cite This Study

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