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January 1, 2008Organic & Biomolecular ChemistryOpen Access

Preparation and purification of 5′-amino-5′-deoxyguanosine-5′-N-phosphoramidate and its initiation properties with T7 RNA polymerase

David Williamson David R. W. Hodgson

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Abstract

5'-Amino-5'-deoxyguanosine-5'-N-phosphoramidate (GNHP) was synthesised in four steps from guanosine. Its identity was confirmed using spectroscopic and kinetic studies. A chromatographic method for the purification of this unstable compound was developed. GNHP was found to initiate T7 RNAP promoted transcriptions to afford 5'-O3P-NH-RNA, which hydrolyses readily to yield 5'-H2N-RNA that can be conjugated to activated esters.

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Williamson et al. (2008) studied this question.

synapsesocial.com/papers/6a963806b93cad5db2d7b151 https://doi.org/10.1039/b717896d

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