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IN spite of a great deal of published work, the present methods of estimation of 1 This coupling must be carried out atpH 3-0-4-0. AbovepH 4-0, the colour of the coupled mix- ture fades rapidly to a greenish tint. At a pH much below 3-0, a permanent blue is obtained (the colour used by many workers, particularly in Germany, for estimation of bilirubin [cf. Krupski & Almasy, 1935]). At pH 3-0-4-0, the maximum amount of red colour is produced
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Haslewood et al. (1937) studied this question.
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