Key result
An immunohistochemical method using polyclonal antiserum detected type 2 porcine circovirus with identical density and distribution of positive cells as in situ hybridization in 18/18 cases.
Absolute Event Rate: 100% vs 100%
The developed immunohistochemical method is a rapid, less expensive, and equally effective alternative to in situ hybridization for detecting PCV2 in porcine tissues.
Supports IHC as equivalent to ISH for PCV2 in limited porcine samples; leaves open prospective validation for routine diagnostic adoption.
An immunohistochemical method for the detection of type 2 porcine circovirus (PCV2) in paraffin-embedded tissue was developed. Rabbits were inoculated with purified PCV2 to obtain a polyclonal antiserum. Antiserum was applied to sections of porcine tissue that contained lesions consistent with postweaning multisystemic wasting syndrome and in which PCV2 genome had been demonstrated by in situ hybridization. In all cases (18/18), the density and distribution of positive cells detected by in situ hybridization or immunohistochemistry were identical. The immunohistochemical method is more rapid and less expensive than in situ hybridization and is thus more suitable for routine diagnostic use.
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Sorden et al. (1999) studied Type 2 porcine circovirus (PCV2) infection (n=18). Immunohistochemical method using polyclonal antiserum vs. In situ hybridization was evaluated on Density and distribution of positive cells. An immunohistochemical method using polyclonal antiserum detected type 2 porcine circovirus with identical density and distribution of positive cells as in situ hybridization in 18/18 cases.
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