The fatty acid composition of carcass and exoskeleton lipids was determined for the freshwater crayfishOrconectes rusticus. Lipid fractions were isolated by column and thin‐layer chromatography. Fatty acid methyl esters and alcohol acetates were then prepared and analyzed by gas‐liquid chromatography. Peak identities were established from retention time data for methyl esters, hydrogenated methyl esters, and saturated, monoene, diene, and polyene methyl esters separated as acetoxy‐mercuri‐methoxy derivatives. Minor component acids were estimated from their relative compositions in these fractions.
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Wolfe et al. (1965) studied this question.
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